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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
18/08/2015 |
Data da última atualização: |
15/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ALEMAN GAÍNZA, Y.; DOMINGUES, L. F.; PINO PEREZ, O.; RABELO, M. D.; ROQUE LÓPEZ, E.; CHAGAS, A. C. de S. |
Afiliação: |
YOUSMEL ALEMAN GAÍNZA, CENSA, MAYABEQUE, CUBA.; LUCIANA FERREIRA DOMINGUES, BOLSISTA, CPPSE, SÃO CARLOS, SP.; ORIELA PINO PEREZ, CENSA, MAYABEQUE, CUBA.; MARCIO DIAS RABELO, CPPSE; EUGENIO ROQUE LÓPEZ, UNIVERSIDAD AGRARIA DE LA HABANA, CUBA.; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
Título: |
Anthelmintic activity in vitro of Citrus sinensis and Melaleuca quinquenervia essential oil from Cuba on Haemonchus contortus. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Industrial Crops and Products, v. 76, p. 647-652, dec. 2015. |
ISSN: |
0926-6690 |
DOI: |
https://doi.org/10.1016/j.indcrop.2015.07.056 |
Idioma: |
Inglês |
Conteúdo: |
Gastrointestinal nematodes are an important problem in the small ruminant production and their control nearly exclusively depends on commercial anthelmintics. However, parasite resistance to those chemical formulations is an increasing global problem, so new plant-derived compounds are being studied for their potential use against gastrointestinal nematodes. Citrus sinensis and Melaleuca quinquenervia essential oils were evaluated against Haemonchus contortus Embrapa2010 resistant isolate, through the egg hatch test (EHT) and larval development test (LDT) at concentrations ranging from 0.02 mg/mL to 50 mg/mL and from 0.04 mg/mL to 3.12 mg/mL, respectively. All concentrations, positive controls (thiabendazole in EHT or ivermectin in LDT), and negative controls (2% Tween 80 in EHT or 0.5% DMSO in LDT) were performed in six replicates and in three independent experiments, for a total of 18 repetitions involving approximately 1800 parasites per treatment. Significant differences (P ? 0.01) of inhibition percentages were identified by one-way analysis of variance followed by the Tukey test. Inhibitory concentration values (IC50 and IC90) were analyzed by the Probit procedure of SAS. The essential oils evaluated showed that C. sinensis presented limonene as major component (96.0%), while M. quinquenervia contained longifolene (32.95%) and 1,8-cineole (25.43%) as major components. In the EHT, the IC50 and IC90 of the essential oils were respectively 0.27 and 0.99 mg/mL for C. sinensis and, 1.52 and 5.63 mg/mL for M. quinquenervia. In the LDT, the IC50 and IC90 were 0.97 and 2.32 mg/mL for C. sinensis and, 0.44 and 0.94 mg/mL for M. quinquenervia. C. sinensis was five times more effective on eggs than M. quinquenervia. However, it was twice more effective on larvae than C. sinensis, indicating that bioactive essential oils have different modes of action. The results suggest that these compounds are good candidates for nematode control. However, alternative anthelmintics have to be safe to the host, promote parasite control in vivo and not generate residues. So, all these key points need to be elucidated before using any plant extract and/or components. MenosGastrointestinal nematodes are an important problem in the small ruminant production and their control nearly exclusively depends on commercial anthelmintics. However, parasite resistance to those chemical formulations is an increasing global problem, so new plant-derived compounds are being studied for their potential use against gastrointestinal nematodes. Citrus sinensis and Melaleuca quinquenervia essential oils were evaluated against Haemonchus contortus Embrapa2010 resistant isolate, through the egg hatch test (EHT) and larval development test (LDT) at concentrations ranging from 0.02 mg/mL to 50 mg/mL and from 0.04 mg/mL to 3.12 mg/mL, respectively. All concentrations, positive controls (thiabendazole in EHT or ivermectin in LDT), and negative controls (2% Tween 80 in EHT or 0.5% DMSO in LDT) were performed in six replicates and in three independent experiments, for a total of 18 repetitions involving approximately 1800 parasites per treatment. Significant differences (P ? 0.01) of inhibition percentages were identified by one-way analysis of variance followed by the Tukey test. Inhibitory concentration values (IC50 and IC90) were analyzed by the Probit procedure of SAS. The essential oils evaluated showed that C. sinensis presented limonene as major component (96.0%), while M. quinquenervia contained longifolene (32.95%) and 1,8-cineole (25.43%) as major components. In the EHT, the IC50 and IC90 of the essential oils were respectively 0.27 and 0.99 mg/mL for C. sinen... Mostrar Tudo |
Palavras-Chave: |
HERBAL MEDICINE. |
Thesagro: |
Citrus Sinensis; Haemonchus Contortus. |
Thesaurus Nal: |
Cuba; essential oils; Melaleuca quinquenervia. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 03069naa a2200277 a 4500 001 2022186 005 2023-03-15 008 2015 bl uuuu u00u1 u #d 022 $a0926-6690 024 7 $ahttps://doi.org/10.1016/j.indcrop.2015.07.056$2DOI 100 1 $aALEMAN GAÍNZA, Y. 245 $aAnthelmintic activity in vitro of Citrus sinensis and Melaleuca quinquenervia essential oil from Cuba on Haemonchus contortus.$h[electronic resource] 260 $c2015 520 $aGastrointestinal nematodes are an important problem in the small ruminant production and their control nearly exclusively depends on commercial anthelmintics. However, parasite resistance to those chemical formulations is an increasing global problem, so new plant-derived compounds are being studied for their potential use against gastrointestinal nematodes. Citrus sinensis and Melaleuca quinquenervia essential oils were evaluated against Haemonchus contortus Embrapa2010 resistant isolate, through the egg hatch test (EHT) and larval development test (LDT) at concentrations ranging from 0.02 mg/mL to 50 mg/mL and from 0.04 mg/mL to 3.12 mg/mL, respectively. All concentrations, positive controls (thiabendazole in EHT or ivermectin in LDT), and negative controls (2% Tween 80 in EHT or 0.5% DMSO in LDT) were performed in six replicates and in three independent experiments, for a total of 18 repetitions involving approximately 1800 parasites per treatment. Significant differences (P ? 0.01) of inhibition percentages were identified by one-way analysis of variance followed by the Tukey test. Inhibitory concentration values (IC50 and IC90) were analyzed by the Probit procedure of SAS. The essential oils evaluated showed that C. sinensis presented limonene as major component (96.0%), while M. quinquenervia contained longifolene (32.95%) and 1,8-cineole (25.43%) as major components. In the EHT, the IC50 and IC90 of the essential oils were respectively 0.27 and 0.99 mg/mL for C. sinensis and, 1.52 and 5.63 mg/mL for M. quinquenervia. In the LDT, the IC50 and IC90 were 0.97 and 2.32 mg/mL for C. sinensis and, 0.44 and 0.94 mg/mL for M. quinquenervia. C. sinensis was five times more effective on eggs than M. quinquenervia. However, it was twice more effective on larvae than C. sinensis, indicating that bioactive essential oils have different modes of action. The results suggest that these compounds are good candidates for nematode control. However, alternative anthelmintics have to be safe to the host, promote parasite control in vivo and not generate residues. So, all these key points need to be elucidated before using any plant extract and/or components. 650 $aCuba 650 $aessential oils 650 $aMelaleuca quinquenervia 650 $aCitrus Sinensis 650 $aHaemonchus Contortus 653 $aHERBAL MEDICINE 700 1 $aDOMINGUES, L. F. 700 1 $aPINO PEREZ, O. 700 1 $aRABELO, M. D. 700 1 $aROQUE LÓPEZ, E. 700 1 $aCHAGAS, A. C. de S. 773 $tIndustrial Crops and Products$gv. 76, p. 647-652, dec. 2015.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Cutter |
Registro |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
13/05/1996 |
Data da última atualização: |
13/05/1996 |
Autoria: |
AVILA, A. C. de. |
Afiliação: |
EMBRAPA-CNPH. |
Título: |
Relatorio de viagem: reuniao nacional de laboratorios de virologia visando a deteccao de virus de batata. |
Ano de publicação: |
1996 |
Fonte/Imprenta: |
Brasilia: EMBRAPA-CNPH, 1996. |
Páginas: |
nao paginado. |
Idioma: |
Português |
Conteúdo: |
Levantamento dos tipos de prestacao de servicos e infra-estrutura que os laboratorios dispoem para deteccao de virus de batata. |
Palavras-Chave: |
Analysis; Batata-semente; Deisease; Deteccao; Laboratory; Seed potato. |
Thesagro: |
Batata; Doença; Elisa; Equipamento; Laboratório; Solanum Tuberosum; Vírus. |
Thesaurus NAL: |
detection; equipment. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00891nam a2200301 a 4500 001 1754829 005 1996-05-13 008 1996 bl uuuu u0uu1 u #d 100 1 $aAVILA, A. C. de 245 $aRelatorio de viagem$breuniao nacional de laboratorios de virologia visando a deteccao de virus de batata. 260 $aBrasilia: EMBRAPA-CNPH$c1996 300 $anao paginado. 520 $aLevantamento dos tipos de prestacao de servicos e infra-estrutura que os laboratorios dispoem para deteccao de virus de batata. 650 $adetection 650 $aequipment 650 $aBatata 650 $aDoença 650 $aElisa 650 $aEquipamento 650 $aLaboratório 650 $aSolanum Tuberosum 650 $aVírus 653 $aAnalysis 653 $aBatata-semente 653 $aDeisease 653 $aDeteccao 653 $aLaboratory 653 $aSeed potato
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